TWSG1
Reactivity: Human
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 488
Application Notes
Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 200 μL sterile PBS and the final concentration is 500 μL/mL.
Buffer
PBS, None
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Twisted gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning in Drosophila and Xenopus by regulating BMP signaling. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. TSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog, and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.Synonyms: Twisted gastrulation protein homolog 1