Podoplanin antibody (PDPN)

Details for Product anti-PDPN Antibody No. ABIN360956, Supplier: Log in to see
Antigen
  • PDPN
  • AGGRUS
  • GP36
  • GP40
  • Gp38
  • HT1A-1
  • OTS8
  • PA2.26
  • T1A
  • T1A-2
  • OTS-8
  • RANDAM-2
  • T1-alpha
  • T1a
  • T1alpha
  • E11
  • RTI40
  • podoplanin
  • podoplanin-like
  • PDPN
  • Pdpn
  • LOC100738269
Alternatives
anti-Rat (Rattus) Podoplanin antibody for ELISA
Reactivity
Rat (Rattus)
292
166
44
5
2
Host
Mouse
132
118
95
45
22
15
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Podoplanin antibody is un-conjugated
27
20
18
17
15
14
11
10
10
10
10
10
9
9
9
8
8
7
5
4
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
304
181
130
120
92
83
70
57
41
14
10
4
3
3
3
3
2
1
1
1
1
Options
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Immunogen BALB/c mice using a membrane protein fraction of isolated Rat glomeruli
Clone LF3(B7)D5B3
Specificity This antibody recognizes Rat Podoplanin.
Purification Protein G Chromatography
Alternative Name Podoplanin (PDPN Antibody Abstract)
Background Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor. It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
Gene ID 54320
NCBI Accession NP_062231
UniProt Q64294
Pathways Dicarboxylic Acid Transport
Application Notes Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 6.0 without preservatives and stabilizers
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for two weeks or (in aliquots) at -20 °C for longer.
Background publications Schacht, Ramirez, Hong, Hirakawa, Feng, Harvey, Williams, Dvorak, Dvorak, Oliver, Detmar: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

Breiteneder-Geleff, Matsui, Soleiman, Meraner, Poczewski, Kalt, Schaffner, Kerjaschki: "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis." in: The American journal of pathology, Vol. 151, Issue 4, pp. 1141-52, 1997 (PubMed).