Apoptotic Peptidase Activating Factor 1 (APAF1) antibody

Antigen: Apoptotic Peptidase Activating Factor 1 (APAF1)

Synonyms: CED4, APAF-1, DKFZp781B1145, fog, Apaf-1, Apaf1l, KIAA0413, mKIAA0413, 6230400I06Rik, ARK, Apaf1, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, arc, dApaf-1/DARK/HAC-1, dApaf1, dapaf, dapaf-1L, dapaf-1S, dark/dapaf-1/hac-1, DmelCG6829, CG6829, apaf-1, MGC80868, APAF1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN361200

Additional Information

Alternative name: Apoptotic peptidase activating factor 1, APAF1

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human APAF1, identical to the related rat and mouse sequence

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23. 1 It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis. 2 This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9. 3 Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death. 4 Additionally, APAF1 has been shown to interact with NLRP1, 5 Caspase-9, 5, 6, 7, 8, 9, 10 APIP, 6 BCL2-like 1 8, 9 and HSPA4. 10

Synonyms: CED4, APAF-1, DKFZp781B1145, fog, Apaf-1, Apaf1l, KIAA0413, mKIAA0413, 6230400I06Rik, ARK, Apaf1, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, arc, dApaf-1/DARK/HAC-1, dApaf1, dapaf, dapaf-1L, dapaf-1S, dark/dapaf-1/hac-1, DmelCG6829, CG6829, apaf-1, MGC80868, APAF1

Application Details

Application Notes: Recommended Dilutions: WB 1ug/ml. IHC-P 2ug/ml. Antigen Retrieval By Heat

Purification: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml. At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time.

Restrictions: For Research Use only

Images

Rat Brain

anti-Apoptotic Peptidase Activating Factor 1 (APAF1) antibody (Image 2)