CD80 (CD80) antibody
| Antigen |
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| Synonyms |
LAB7, CD28LG, CD28LG1 |
| Clonality |
Monoclonal (8-E5) |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (188), Western Blotting (WB) (52), Immunofluorescence (IF) (49), Immunoprecipitation (IP) (48), ELISA (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Functional Studies (Func) (16), Immunohistochemistry (IHC) (12), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN361246 |
| Quantity |
100ul (0.32mg/ml) |
| Price |
350.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Positive control: Brain tissue.
Cellular Localization: Cell membrane
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Alternative name
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CD80/B7-1
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Swiss-Prot
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P33681
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Immunogen
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This antibody is produced by immunizing mouse with a synthetic peptide (KLH-coupled) corresponding to CD80.
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Cross-Reactivity
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Human
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Format
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Liquid
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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8-E5
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Description
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B7-1 is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines. B7-1 is a costimulatory molecule for the activation of both CD4+ and CD8+ T lymphocytes that prevents the induction of clonal anergy. Thus, the transfer of B7-1 genes into tumor cells can induce protective immunity and lead to tumor rejection of some tumors in model systems of in vivo tumor growth.
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Specificity
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Cellular Localization: Cell membrane. Molecular Weight: 60kDa
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Molecular Weight
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60kDa
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Application Notes
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WB: 1:500
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Concentration
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0.32mg/ml
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Buffer
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1xTBS (pH7.4), 0.5%BSA, 25%Glycerol.
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Preservative
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0.05% Sodium Azide.
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Storage
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Store at +4° C after thawing. Aliquot store at -20° C or -80° C. Avoid repeated freeze / thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD80 (CD80)", type "Antibodies"
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Hosts
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Mouse (110), Rat (61), Rabbit (53), Hamster (18), Goat (2)
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Reactivities
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Human (114), Mouse (Murine) (110), Rat (Rattus) (29), Chicken (6), Dog (Canine) (4), Rabbit (3), Baboon (2), Cow (Bovine) (2), Horse (Equine) (2), Pig (Porcine) (1)
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Applications
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Flow Cytometry (FACS) (188), Western Blotting (WB) (52), Immunofluorescence (IF) (49), Immunoprecipitation (IP) (48), ELISA (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Functional Studies (Func) (16), Immunohistochemistry (IHC) (12), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Neutralization (Neut) (1)
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Conjugates
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FITC (27), PE (27), Biotin (26), APC (8), Cy5,PE (6), PE,Cy5 (4), Alexa Fluor 488 (3), RPE (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
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Epitopes
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AA 45-63 (1), extra-cellular domain (1)
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Alternatives 
