Deoxynucleotidyltransferase, terminal (DNTT) (C-Term near) antibody

Antigen: Deoxynucleotidyltransferase, terminal (DNTT)

Synonyms: TDT, Tdt, BB160593, DNTT, MGC109152, dntt-A, TdT

Epitope: C-Term near

Clonality: Monoclonal (B4-F5)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN361250

Additional Information

Alternative name: TdT C-term near

Swiss-Prot: P04053

Immunogen: This antibody is produced by immunizing mouse with a synthetic peptide (KLH-coupled) corresponding to near C-terminal residues of TdT.

Format: Liquid

Clone: B4-F5

Description: Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase expressed in immature lymphocytes of the thymus and bone marrow, as well as certain leukemic cells. The nuclear enzyme TdT plays a critical role in generating immune receptor diversity. During rearrangement of Ig and TCR V region genes, TdT catalyzes the addition of N nucleotides without a DNA template to V-D and D-J junctions in both Ig and TCR as well as the V-J junctions in the TCR (1–3). During the fetal and newborn period, TdT activity is absent, resulting in an N region-deficient Ag receptor repertoire significantly lacking in the diversity observed in the junctional regions of Igs and TCRs of adult animals Background

Specificity: Cellular Localization: Mitochondrion. Molecular Weight: 58kDa

Application Details

Application Notes: Recommended Dilutions: WB: 1:1,000. Positive Control: Jurkat cells

Concentration: 0.43mg/ml

Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.

Storage: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Restrictions: For Research Use only

Images

Western blot analysis on jurkat lysates using anti-TdT monoclonal antibody.