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Actin alpha antibody

Antigen

Actin alpha

Synonyms Actc-1
Clonality Monoclonal (33-32)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Application
Alternatives Western Blotting (WB)
Catalog no. ABIN361252
Quantity 100ul  (0.5mg/ml)
Price 350.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Characteristics Positive control: Mouse Heart.
Cellular Localization: Cytoplasma
Alternative name alpha-Actin
Swiss-Prot P68033/P68032
Immunogen This antibody is produced by immunizing mouse with a synthetic peptide (KLH-coupled) corresponding to near N-terminal residues of alpha-Actin.
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Format Liquid
Isotype IgG1  (Matching secondary antibodies)
Clone 33-32
Description Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells, whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis
Specificity Cellular Localization: Cytoplasma. Molecular Weight: 42kDa
Molecular Weight 42kDa

Application Details

Application Notes WB: 1:5,000
Concentration 0.5mg/ml
Buffer 1xTBS (pH7.4), 0.5%BSA, 25%Glycerol.
Preservative 0.05% Sodium Azide.
Storage Store at +4° C after thawing. Aliquot store at -20° C or -80° C. Avoid repeated freeze / thaw cycles.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Actin alpha", type "Antibodies"
Hosts Rabbit (4)
Reactivities Human (2), Mouse (Murine) (2), Rat (Rattus) (2)
Applications Western Blotting (WB) (4), ELISA (1), Immunohistochemistry (IHC) (1)