Lactate Dehydrogenase A (LDHA) antibody

Antigen: Lactate Dehydrogenase A (LDHA)

Synonyms: LDH1, LDHM, GSD11, PIG19, LDHA, LDH-M, Ldh1, Ldhm, l7R2, ldhbb, ldha-B, MGC83980, ldh1, ldhm, gsd11, ldh-m, pig19, MGC53139, MGC76171, MGC90004, ldha1, ldhab, LDH-A, LDHC, DKFZp469J2231

Clonality: Monoclonal (20-179)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN361268

Additional Information

Characteristics: Positive control: A549.
Cellular Localization: Cytoplasm

Alternative name: LDH-A

Swiss-Prot: P00338

Immunogen: This antibody is produced by immunizing mouse with a synthetic peptide (KLH-coupled) corresponding to near N-terminal residues of LDH-A.

Cross-Reactivity: Human, Mouse (Murine)

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 20-179

Description: Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.

Specificity: Cellular Localization: Cytoplasm. Molecular Weight: ~35kDa

Molecular Weight: ~35kDa

Application Details

Application Notes: WB: 1:500

Concentration: 0.5mg/ml

Buffer: 1xTBS (pH7.4), 0.5%BSA, 25%Glycerol.

Preservative: 0.05% Sodium Azide.

Storage: Store at +4° C after thawing. Aliquot store at -20° C or -80° C. Avoid repeated freeze / thaw cycles.

Restrictions: For Research Use only

Images

Western blot analysis on A549 using anti-LDH-A monoclonal antibody