ATP6V0D2 antibody (Isoform 1)

Catalog No. ABIN361273

Product Details anti-ATP6V0D2 Antibody

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Binding Specificity: Isoform 1

Reactivity: Tomato, Arabidopsis thaliana, Nicotiana tabacum

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP6V0D2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Specificity has been determined using ELISA competition experiments using conjugate of 5-Methoxytryptamine-Glutaric anhydride Compounds Cross-reactivity ratio (a) 5-Methoxytryptamine-S-BSA: 1, Free melatonin 1/ 130, 5-Hydroxytryptamine-A-BSA 1/ 50 000, DL 5-Methoxytryptophan-SA-BSA 1/>50000, Tryptamine-SA-BSA 1/>20 000 (a) : 5-Methoxytryptamine-GA-BSA concentration/ conjugated close structure concentration at half displacement.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 26 / 31 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Application Details

Application Notes: Recommended Dilution: 1 : 1 000 with alkaline phosphatase or 1 : 3000 with ECL (WB).

Comment:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 300 µL of sterile water.

Handling Advice: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Target Details for ATP6V0D2

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Alternative Name: V-ATPase (ATP6V0D2 Products)

Synonyms: ATP6D2 antibody, VMA6 antibody, AI324824 antibody, V-ATPase antibody, 1620401A02Rik antibody, ATPase H+ transporting V0 subunit d2 antibody, ATPase, H+ transporting, lysosomal V0 subunit D2 antibody, ATPase H+ transporting V0 subunit D2 antibody, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 L homeolog antibody, ATP6V0D2 antibody, Atp6v0d2 antibody, atp6v0d2.L antibody

Background: AGI Code: At4g11150
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Molecular Weight: expected: 26 kDa, apparent: 31 kDa (Arabidopsis thaliana)

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport