Transforming Protein antibody

Antigen: Transforming Protein

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB)

Catalog no.: ABIN361294

Additional Information

Alternative name: E7 transforming protein [Human papillomavirus type 16]

Gene ID: 1489079

Immunogen: purified recombinant E7 HPV16

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Application Details

Application Notes: E7 HPV16 antibody has been tested for use in Western Blotting. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Purity: > 95 % SDS

Buffer: PBS + 0,1% NaN3

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Research Area: Virology

Restrictions: For Research Use only

Images

Western Blotting analysis of recombinant protein E7 produced in Escherichia coli BL21 (lambdaDE3) transfected bacterial culture. SDS-PAGE Electrophoresis (reducing conditions).Immunostaining with rabbit polyclonal antibody to E7 – HPV16, dilution 0,5 µg/ml (3, 4) non purified serum (1, 2). Detection with goat anti-rabbit / HRP secondary antibody. Lane 2, 4: Escherichia coli transfected bacterial culture (cell lysate). Lane 1, 3: Escherichia coli non-transfected bacterial culture (cell lysate) - negative controls.