Ag85B antibody

Antigen: Ag85B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Bacteria

Application: Western Blotting (WB)

Catalog no.: ABIN361295

Additional Information

Gene ID: 29027589

Immunogen: purified recombinant Ag85B

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Application Details

Application Notes: Ag85B antibody has been tested for use in Western Blotting. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Purity: > 95 % SDS

Buffer: PBS + 0,1% NaN3

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Western Blotting analysis of recombinant protein Ag85B produced in Escherichia coli BL21 (lambdaDE3) transfected bacterial culture. SDS-PAGE Electrophoresis (Reducing conditions).Immunostaining with rabbit polyclonal antibody to Ag85B, dilution 1,0 µg/ml (3, 4), non purified serum (1, 2). Detection with goat anti-rabbit / HRP secondary antibody. Lane 1, 3: Escherichia coli non-transfected bacterial culture (cell lysate) - negative controls. Lane 2, 4: Escherichia coli transfected bacterial culture (cell lysate).