Interferon gamma (IFNG) (C-Term peptide) antibody

Antigen: Interferon gamma (IFNG)

Synonyms: IFG, IFI, Ifg, IFN-g, IFNG2, IFNG, IFN-gamma

Epitope: C-Term peptide

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN361307

Additional Information

Alternative name: Interferon gamma (IFN gamma) C-terminal peptide

Gene ID: 3458

Immunogen: IFNg C-term peptide

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Application Details

Application Notes: IFNg C-term peptide antibody has been tested for use in Western Blotting and ELISA. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Purity: > 95 % SDS

Buffer: PBS + 0,1% NaN3

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Research Area: Cytokines

Restrictions: For Research Use only

Images

Western Blotting analysis of recombinant protein h-IFN gamma produced in Escherichia coli BL21 ((DE3) transfected bacterial culture. SDS-PAGE Electrophoresis (Reducing conditions). Rabbit was immunized with syntethic C-terminal peptide derived from IFN gamma.Immunostaining with rabbit polyclonal antibody to h-IFN gamma, dilution 1 µg/ml (1, 2), non purified serum (3, 4). Detection with goat anti-rabbit / HRP secondary antibody. Lane 1, 3: Escherichia coli non-transfected bacterial culture (cell lysate) - negative controls. Lane 2, 4: Escherichia coli transfected bacterial culture (cell lysate).