Early Secretory Antigenic Target ESXA (ESAT-6) antibody

Antigen: Early Secretory Antigenic Target ESXA (ESAT-6)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Bacteria

Application: Western Blotting (WB)

Catalog no.: ABIN361308

Additional Information

Gene ID: 886209

Immunogen: purified recombinant ESAT-6

Format: Affinity-purified

Isotype: IgG

Application Details

Application Notes: ESAT-6 antibody has been tested for use in Western Blotting. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Purity: > 95 % SDS

Purification: DEAE Sepharose, IMAC-Talon® resins.

Buffer: PBS + 0,1% NaN3

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Western Blotting analysis of recombinant protein ESAT-6 produced in Escherichia coli BL21 (lambdaDE3) transfected bacterial culture. SDS-PAGE Electrophoresis (Reducing conditions).Immunostaining with rabbit polyclonal antibody to ESAT6, dilution 0,5 µg/ml (3, 4), non purified serum (1, 2). Detection with goat anti-rabbit / HRP secondary antibody. Lane 1, 3: Escherichia coli non-transfected bacterial culture (cell lysate) - negative controls. Lane 2, 4: Escherichia coli transfected bacterial culture (cell lysate).