PU1 peptide antibody

Antigen: PU1 peptide

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), Confocal Microscopy (CM)

Catalog no.: ABIN361312

Additional Information

Gene ID: 19845

Immunogen: PU1 peptide

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Application Details

Application Notes: PU1 peptide antibody has been tested for use in Western Blotting and confocal microscopy. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Purity: > 95 % SDS

Buffer: PBS + 0,1% NaN3

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Detection of PU.1 and PU.1-ER using the anti-PU.1 antibodies on WB. Protein lysates were prepared by the RIPA buffer lysis of 1x106 Murine erythroleukemia (MEL) cells that express PU.1 (M) or murine hematopoietic progenitor cells isolated from PU.1 knockout mice and expressing transgenic PU.1-ER (P). SDS-PAGE were run and blotted on membranes and incubated with four different anti-PU.1 antibodies (37, 38, 39, 40) in a dilution 1:500 in PBS-Tween20 solution. Secondary antibody (anti-rabbit linked with horse reddish peroxidase) was used in a dilution 1:4000 followed by luminescence detection on X-ray film. Figure 2. Immunofluorescence of PU.1 in MEL cells with anti-PU.1 antibody (R-037-AI-01, dilution 1:100 in 1% BSA-PBS). Cells were fixed in 3.5% paraformaldehyde in PBS, permeabilized with 0.5% Triton X-100 in PBS and blocked using 3% non-fat milk in PBS. Secondary antibody (Alexa Fluor 488, Invitrogen, cat.# A-11070) was used in a dilution 1:200 in 2% BSA-PBS. DNA was counterstained with DAPI