H-Calreticulin (HCRT) antibody

Antigen: H-Calreticulin (HCRT)

Synonyms: RO, CRT, SSA, cC1qR, FLJ26680, Calregulin, zgc:85636, Clr, anon-EST:Liang-2.11, anon-EST:Liang-2.17, anon-EST:fe3D7, clone 2.11, clone 2.17, eth, potp, DmelCG9429, CG9429, crc, MGC52637, crt, ssa, cc1qr, MGC69541, CALR, Crt, NV19007

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN361313

Additional Information

Gene ID: 811

Immunogen: purified recombinant hCRT

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Synonyms: RO, CRT, SSA, cC1qR, FLJ26680, Calregulin, zgc:85636, Clr, anon-EST:Liang-2.11, anon-EST:Liang-2.17, anon-EST:fe3D7, clone 2.11, clone 2.17, eth, potp, DmelCG9429, CG9429, crc, MGC52637, crt, ssa, cc1qr, MGC69541, CALR, Crt, NV19007

Application Details

Application Notes: hCRT antibody has been tested for use in Western Blotting. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Purity: > 95 % SDS

Purification: DEAE Sepharose, IMAC-Talon® resins.

Buffer: PBS + 0,1% NaN3

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Western Blotting analysis of recombinant protein h-CRT produced in Escherichia coli BL21 (lambdaDE3) transfected bacterial culture. SDS-PAGE Electrophoresis (Reducing conditions).Immunostaining with rabbit polyclonal antibody to h-CRT, dilution (1, 2) 0,5 µg/ml. Detection with goat anti-rabbit / HRP secondary antibody. Lane 2,: Escherichia coli transfected bacterial culture (cell lysate). Lane 1: Escherichia coli non-transfected bacterial culture (cell lysate) - negative control.