RFP antibody

Antigen: RFP

Clonality: Polyclonal

Host: Rabbit

Reactivity: Bacteria

Application: Western Blotting (WB)

Catalog no.: ABIN361315

Additional Information

Gene ID: 53748886

Immunogen: purified recombinant RFP

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Application Details

Application Notes: RFP antibody has been tested for use in Western Blotting. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Purity: > 95 % SDS

Purification: DEAE Sepharose, IMAC-Talon® resins.

Buffer: PBS + 0,1% NaN3

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Western Blotting analysis of recombinant protein RFP produced in Escherichia coli BL21 ((DE3) transfected bacterial culture. SDS-PAGE Electrophoresis (Reducing conditions).Immunostaining with rabbit polyclonal antibody to RFP , dilution (1, 2) 1 µg/ml, non purified serum (3, 4). Detection with goat anti-rabbit / HRP secondary antibody. Lane 1, 3: Escherichia coli non-transfected bacterial culture (cell lysate) - negative controls. Lane 2, 4: Escherichia coli transfected bacterial culture (cell lysate).