Enhanced Green Fluorescent Protein (eGFP) antibody

Antigen: Enhanced Green Fluorescent Protein (eGFP)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Bacteria

Application: Western Blotting (WB)

Catalog no.: ABIN361317

Additional Information

Alternative name: EGFP

Gene ID: 8382257

Immunogen: purified recombinant EGFP

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Application Details

Application Notes: EGFP antibody has been tested for use in Western Blotting. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Purity: > 95 % SDS

Purification: Immobilized Metall Affinity Chromatography (IMAC)-Talon® resins.

Buffer: PBS + 0,1% NaN3

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Western Blotting analysis of recombinant protein EGFP produced in Escherichia coli BL21 ((DE3) transfected bacterial culture. SDS-PAGE Electrophoresis (Reducing conditions).Immunostaining with rabbit polyclonal antibody to EGFP, dilution 1 µg/ml (1, 2) non purified serum (3, 4). Detection with goat anti-rabbit / HRP secondary antibody. Lane 1, 3: Escherichia coli transfected bacterial culture (cell lysate). Lane 2, 4: Escherichia coli non-transfected bacterial culture (cell lysate) - negative controls.