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Antibodies
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anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody
Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody
| Antigen |
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| Synonyms |
MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (88), Rat (Rattus) (65), Mouse (Murine) (60), Cow (Bovine) (19), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (17), Chicken (2), Guinea Pig (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Application
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Western Blotting (WB) (72), Immunofluorescence (IF) (55), Flow Cytometry (FACS) (20), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (10), Immunocytochemistry (ICC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN361346 |
| Quantity |
100 µl (Variants) |
| Price |
295.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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MARCKS
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Description
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Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al. 1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994). Anti- MARCKS Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~80k MARCKS protein. Right: Mixed neuron/glial cultures stained with MARCKS antibody (red) anti-MAP2 (green), catalog 1100-MAP2.
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Specificity
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Specific for the ~80k MARCKS protein. It is expected that it will work on other mammalian tissues.
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Application Notes
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Recommended Dilution: WB: 1:10,000 IF: 1:500 Quality Control: Western blots performed on each lot.
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Purification
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Unpurified neat serum.
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Storage
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Store at -20 °C, stable for 1 year
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Research Area
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Cardiovascular
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Restrictions
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For Research Use only
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Alternatives for antigen "Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)", type "Antibodies"
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Hosts
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Rabbit (96), Mouse (7), Rat (2)
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Reactivities
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Human (88), Rat (Rattus) (65), Mouse (Murine) (60), Cow (Bovine) (19), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (17), Chicken (2), Guinea Pig (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Western Blotting (WB) (72), Immunofluorescence (IF) (55), Flow Cytometry (FACS) (20), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (10), Immunocytochemistry (ICC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer158 (7), pSer162 (7), pSer170 (6), Ab-162 (4), C-Term (2), N-Term (2), pSer152, pSer156 (1), pSer152/156 (1), pSer163 (1), phospho (1)
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Alternatives 
