Enolase 2 (Gamma, Neuronal) (ENO2) antibody

Antigen: Enolase 2 (Gamma, Neuronal) (ENO2)

Synonyms: ENO2, DKFZp459B1817, NSE, Eno-2, AI837106, D6Ertd375e, RNEN3, Lrpb7

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN361357

Additional Information

Alternative name: Neuron Specific Enolase (NSE)

Description: Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).

Specificity: Specific for the ~47kDa NSE protein. It is expected that it will work on other mammalian tissues.

Application Details

Application Notes: Recommended Dilution: WB: 1:2,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Purification: Unpurified neat serum.

Storage: Store at -20 °C, stable for 1 year

Research Area: Cell/Tissue Markers, Cancer

Restrictions: For Research Use only

Images

Western blot of rat cortex homogenate showing specific immunolabeling of the ~ 47k NSE protein.

HEK 293 cells showing staining with anti-NSE antibody (red). The green channels shows staining for UCHL1.