Human Nuclei - Excellent marker for human cells in xenographic research antibody

Antigen: Human Nuclei - Excellent marker for human cells in xenographic research

Clonality: Monoclonal (235-1)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN361360

Additional Information

Immunogen: Human cell homogenate.

Isotype: IgG1  (Matching secondary antibodies)

Clone: 235-1

Description: This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.

Specificity: The antibody has been directly tested for reactivity in human. This antibody does not react with mouse or rat nuclei. Specific for human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

Comments:

Normally ABIN361360 should be fix with 10% buffered formalin overnight at room temperature instead of 4% paraformaldehyde. 1.Use 10% buffered formalin overnight instead of 4% paraformaldehyde to fix the tissues 2.Quench the endogenous peroxidase activity with 3% H2O2 instead of 0.5% H2O2 3.Block non-specific binding sites with 3% horse serum instead of goat serum.

Application Details

Application Notes: Recommended Dilution: IF: 1:100 IHC: 1: 100. Quality Control: Immunofluorescence performed on each lot.

Purity: culture supernatant

Purification: culture supernatant.

Buffer: culture supernatant. Liquid in PBS.

Storage: Store at –20°C in undiluted aliquots. Stable for up to one year after date of receipt. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunostaining of HeLa cells showing specific labeling of their nuclei using our anti-nuclei antibody.