Fractin A Marker of Apoptosis antibody

Antigen: Fractin A Marker of Apoptosis

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361376

Additional Information

Description: Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer?s disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press). Anti-Fractin Western blot of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~ 32k cleaved actin fragment (fractin).

Specificity: Specific for the ~ 32 kDa fractin protein in Western blots with no reactivity to intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases.

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IHC: 1:100 Quality Control: Western blots performed on each lot.

Purification: neat serum.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~ 32k cleaved actin fragment (fractin).