Tryptophan Hydroxylase 1 (TPH1) antibody

Antigen: Tryptophan Hydroxylase 1 (TPH1)

Synonyms: tph, tph1, tphD1, SERGEF, TPH1, TPH2, TPRH, TRPH, MGC119994, Tph, TPH, LOC100229598

Clonality: Polyclonal

Host: Sheep

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN361412

Additional Information

Alternative name: Tryptophan Hydroxylase

Format: Affinity-purified

Description: Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001). Anti-Tryptophan Hydroxylase Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.

Specificity: Specific for the ~55k tryptophan hydroxylase protein.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, Haycock et al., 2002): 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from sheep serum by affinity purification using a column to which the recombinant protein was coupled.

Storage: Store at -20 °C, stable for 1 year

Research Area: Cancer, Amino Acids, Metabolism, Enzymes

Restrictions: For Research Use only

Images

Western blots of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.