Metabotropic Glutamate Receptor 5/1a antibody

Antigen: Metabotropic Glutamate Receptor 5/1a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN361417

Additional Information

Format: Affinity-purified

Description: The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004, Francesconi et al., 2004, Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004). Anti-mGluR5/1a Western blot of 10 ug of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125k monomer and the ~250k dimers of mGluR1a and mGluR5.

Specificity: Specific for the ~125k monomer and the ~250k dimers of mGluR5 and mGluR1. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:500 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of 10 ug of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125k monomer and the ~250k dimers of mGluR1a and mGluR5.