GABAA Receptor, A4-Subunit (N-Term) antibody

Antigen: GABAA Receptor, A4-Subunit

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361441

Additional Information

Alternative name: GABAA Receptor, a4-Subunit, N-Terminus

Format: Affinity-purified

Description: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six (s, four (s and four (s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for (- and (-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a (-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different (-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003). Anti-GABAA-Receptor, a4-Subunit Western blot of 10 (g of rat brain hippocampus (Hipp) lysate showing immunolabeling of the ~64k a4-subunit of the GABAA -R.

Specificity: Specific for the ~64k a4-subunit of the GABAA receptor in Western blots of rat brain extracts.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of 10 (g of rat brain hippocampus (Hipp) lysate showing immunolabeling of the ~64k a4-subunit of the GABAA -R.

Publications

Publications: Diaz, Christian, Anderson et al.: "Chronic ethanol and withdrawal differentially modulate lateral/basolateral amygdala paracapsular and local GABAergic synapses." in: The Journal of pharmacology and experimental therapeutics, Vol. 337, Issue 1, pp. 162-70, 2011 (PubMed).