GABAA Receptor, ß3-Subunit antibody

Antigen: GABAA Receptor, ß3-Subunit

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN361448

Additional Information

Format: Affinity-purified

Description: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six (s, four (s and four (s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for (- and (-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a (-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different (-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003). Anti-GABAA-Receptor, ß3-Subunit Western blot of 5-7 ug of mouse cerebellum lysates from wild type (Control) and (3 knockout ((3 K/O) animals showing specific immunolabeling of the ~53k (3-subunit of the GABAA-R in the wild type but not in the (3 K/O animals.

Specificity: Specific for the ~53k ß3-subunit of the GABAA receptor in Western blots.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of 5-7 ug of mouse cerebellum lysates from wild type (Control) and (3 knockout ((3 K/O) animals showing specific immunolabeling of the ~53k (3-subunit of the GABAA-R in the wild type but not in the (3 K/O animals.

Publications

Publications: Porcher, Hatchett, Longbottom et al.: "Positive feedback regulation between gamma-aminobutyric acid type A (GABA(A)) receptor signaling and brain-derived neurotrophic factor (BDNF) release in developing neurons." in: The Journal of biological chemistry, Vol. 286, Issue 24, pp. 21667-77, 2011 (PubMed).