GABAA Receptor, D-Subunit (N-Term) antibody

Antigen: GABAA Receptor, D-Subunit

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN361449

Additional Information

Alternative name: GABAA Receptor, d-Subunit, N-Terminus

Format: Affinity-purified

Description: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six (s, four (s and four (s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for (- and (-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a (-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different (-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, P ltl et al., 2003). More recently there have been a number of studies demonstrating that the d-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003, Wohlfarth et al., 2002). Anti-GABAA Receptor, d-Subunit Western blot of mouse cerebellar lysates from wild type (Control) and d-knockout (d-K/O) animals showing specific immunolabeling of the ~52k d-subunit of the GABAA-R. The labeling was absent from a lysate prepared from d-knockout animals.

Specificity: Specific for the ~52k d-subunit of the GABAA receptor.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, Korpi et al. 2002): 1:250 IP: 10 µl per 50 µg lysate Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of mouse cerebellar lysates from wild type (Control) and d-knockout (d-K/O) animals showing specific immunolabeling of the ~52k d-subunit of the GABAA-R. The labeling was absent from a lysate prepared from d-knockout animals.

Publications

Publications: Uusi-Oukari, Kontturi, Coffey et al.: "AMPAR signaling mediating GABA(A)R delta subunit up-regulation in cultured mouse cerebellar granule cells." in: Neurochemistry international, 2010 (PubMed).

Sarkar, Wakefield, MacKenzie et al.: "Neurosteroidogenesis is required for the physiological response to stress: role of neurosteroid-sensitive GABAA receptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 50, pp. 18198-210, 2011 (PubMed). (1:500).