Calcium/Calmodulin-Dependent Protein Kinase Type II (CAMK2) (pThr305) antibody

Antigen: Calcium/Calmodulin-Dependent Protein Kinase Type II (CAMK2)

Synonyms: CAMK, CAMKG, CAMK-II, FLJ16043, MGC26678

Epitope: pThr305

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361459

Additional Information

Alternative name: Phospho-Thr305 CaM Kinase II

Description: Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998, Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003, Soderling, 2000, Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003, Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity of CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002). Anti-Phospho Thr305 CaM Kinase II Western blot of rat brain lysate showing specific immunolabeling of the ~50k (- and the ~60k (-CaM Kinase II phosphorylated at Thr305 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Peptide) but not by the corresponding dephosphopeptide (not shown).

Specificity: Specific for the ~50k a-CaM Kinase II and the ~60k ß-CaM Kinase II proteins phosphorylated at Thr305. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Unpurified neat serum.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat brain lysate showing specific immunolabeling of the ~50k (- and the ~60k (-CaM Kinase II phosphorylated at Thr305 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Peptide) but not by the corresponding dephosphopeptide (not shown).