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Description
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-catenin is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway (Ding and Dale, 2002, Polakis, 2000). The role of -catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37 and Thr41 by glycogen synthase kinase 3 (GSK3 ) (Liu et al., 2002). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of -catenin. Mutations in components of this phosphorylation regulated process that prevent -catenin hyperphosphorylation by GSK3 are strongly associated with cancers (Wang et al., 2003, Polakis, 2000, Liu et al., 2002). Anti-Phospho-Ser33,37 -Catenin Western blot of human embryonic kidney cell (HEK) lysate showing specific immunolabeling of the ~83k -catenin phosphorylated at Ser33 and Ser37 (Upper Panel) and a pan -catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3 knocked down by SiRNA.
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