Syntaxin Binding Protein 1 (STXBP1) (pSer515) antibody

Antigen: Syntaxin Binding Protein 1 (STXBP1)

Synonyms: EIEE4, UNC18, RBSEC1, FLJ37475, MUNC18-1, Ms10g, nsec1, N-sec1, Sxtbp1, Unc18h, MMS10-G, Rb-sec1, Unc18-1, AI317162, AI326233, Munc-18a, Munc18-1, MGC140579, stxbp1, MGC88906, fj43h10, wu:fj36d12, wu:fj43h10, zgc:114171, rop, unc18, hunc18, rbsec1, munc18-1, MGC146482

Epitope: pSer515

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361480

Additional Information

Alternative name: Phospho-Ser515 Munc-18

Format: Affinity-purified

Description: Munc-18 ( mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996). Anti-Phospho-Ser515 Munc-18 Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown). .

Specificity: Specific for the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Synonyms: EIEE4, UNC18, RBSEC1, FLJ37475, MUNC18-1, Ms10g, nsec1, N-sec1, Sxtbp1, Unc18h, MMS10-G, Rb-sec1, Unc18-1, AI317162, AI326233, Munc-18a, Munc18-1, MGC140579, stxbp1, MGC88906, fj43h10, wu:fj36d12, wu:fj43h10, zgc:114171, rop, unc18, hunc18, rbsec1, munc18-1, MGC146482

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown). .