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GRIN2B antibody (pTyr1472)

GRIN2B Reactivity: Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN361485
  • Target See all GRIN2B Antibodies
    GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
    Binding Specificity
    • 33
    • 30
    • 24
    • 23
    • 22
    • 20
    • 16
    • 10
    • 9
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr1472
    Reactivity
    • 195
    • 181
    • 152
    • 30
    • 27
    • 24
    • 10
    • 10
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Rat
    Host
    • 205
    • 38
    • 1
    Rabbit
    Clonality
    • 207
    • 37
    Polyclonal
    Conjugate
    • 107
    • 11
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This GRIN2B antibody is un-conjugated
    Application
    • 197
    • 66
    • 65
    • 65
    • 46
    • 41
    • 32
    • 24
    • 24
    • 18
    • 16
    • 11
    • 10
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. The antibody also labels prote~65k and ~115k. Immunolabeling is completely blocked by blocked by either (-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
    Cross-Reactivity
    Mouse (Murine), Rat (Rattus)
    Predicted Reactivity
    bovine, canine, chicken, human, non-human primate, zebra fish
    Purification
    Antigen Affinity Purified from Pooled Serum
    Immunogen
    Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 conjugated to KLH
    Top Product
    Discover our top product GRIN2B Primary Antibody
  • Application Notes
    Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
    Storage
    -20 °C
  • Knox, Brennan-Minnella, Lu, Yang, Nakazawa, Yamamoto, Swanson, Ferriero, Jiang: "NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia." in: Stroke; a journal of cerebral circulation, Vol. 45, Issue 10, pp. 3040-7, (2014) (PubMed).

    Gladding, Sepers, Xu, Zhang, Milnerwood, Lombroso, Raymond: "Calpain and STriatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington's disease mouse model." in: Human molecular genetics, Vol. 21, Issue 17, pp. 3739-52, (2012) (PubMed).

    Castillo, Norcini, Baquero-Buitrago, Levacic, Medina, Montoya-Gacharna, Blanck, Dubois, Recio-Pinto: "The NMDA-evoked cytoplasmic calcium increase in adult rat DRG neuronal somata was potentiated by SP pretreatment in a PKC-dependent manner." in: Neuroscience, (2011) (PubMed).

    Carreño, Walch, Dutta, Nedungadi, Cunningham: "Brain-Derived Neurotrophic Factor-Tyrosine Kinase B Pathway Mediates NMDA Receptor NR2B Subunit Phosphorylation in the Supraoptic Nuclei Following Progressive Dehydration." in: Journal of neuroendocrinology, Vol. 23, Issue 10, pp. 894-905, (2011) (PubMed).

    Venkitaramani, Moura, Picciotto, Lombroso: "Striatal-enriched protein tyrosine phosphatase (STEP) knockout mice have enhanced hippocampal memory." in: The European journal of neuroscience, Vol. 33, Issue 12, pp. 2288-98, (2011) (PubMed).

    Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).

  • Target
    GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
    Alternative Name
    GRIN2B (GRIN2B Products)
    Synonyms
    NMDAR2B antibody, GluN2B antibody, MRD6 antibody, NR2B antibody, hNR3 antibody, nmdar2b antibody, nr2b antibody, AW490526 antibody, Nmdar2b antibody, glutamate ionotropic receptor NMDA type subunit 2B antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 2B L homeolog antibody, glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody, GRIN2B antibody, Grin2b antibody, grin2b.L antibody
    Background
    The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a, Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001, Roche et al., 2001). Anti-Phospho Tyr1472 NMDA NR2B-Subunit Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.
    Molecular Weight
    '180 kDa
    Gene ID
    24410
    UniProt
    Q00960
    Pathways
    Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
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