Tryptophan Hydroxylase 2 antibody (pSer19)

Catalog No. ABIN361498

Product Details anti-Tryptophan Hydroxylase 2 Antibody

Target: Tryptophan Hydroxylase 2 (TPH2)

Binding Specificity: pSer19

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.

Cross-Reactivity: Rat (Rattus)

Predicted Reactivity: bovine, mouse, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-Tryptophan Hydroxylase 2 antibody (ABIN361498)

Matsuo, Izumi, Wakita, Kume, Takada-Takatori, Sawada, Akaike: "Haloperidol, spiperone, pimozide and aripiprazole reduce intracellular dopamine content in PC12 cells and rat mesencephalic cultures: implication of inhibition of vesicular transport." in: European journal of pharmacology, (2010) (PubMed).

Target Details for Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Alternative Name: TPH2 (TPH2 Products)

Synonyms: ADHD7 antibody, NTPH antibody, Ntph antibody, tphR antibody, wu:fq15a04 antibody, AU043594 antibody, tryptophan hydroxylase 2 antibody, tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) antibody, TPH2 antibody, Tph2 antibody, tph2 antibody

Background: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005). Anti-Phospho Ser19 Tryptophan Hydroxylase 2 Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.

Molecular Weight: '55 kDa

Gene ID: 317675

UniProt: Q8CGU9