Tryptophan Hydroxylase 1 (TPH1) (pSer58) antibody

Antigen: Tryptophan Hydroxylase 1 (TPH1)

Synonyms: tph, tph1, tphD1, SERGEF, TPH1, TPH2, TPRH, TRPH, MGC119994, Tph, TPH, LOC100229598

Epitope: pSer58

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rabbit

Application: Western Blotting (WB)

Catalog no.: ABIN361500

Additional Information

Alternative name: Phospho-Ser58 Tryptophan Hydroxylase 1

Format: Affinity-purified

Description: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997). Anti-Phospho Ser58 Tryptophan Hydroxylase Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.

Specificity: Specific for the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.