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tyrosine Hydroxylase (TH) (pSer40) antibody

Details for Product No. ABIN361503, Supplier: Log in to see
Antigen
  • CG10118
  • DH65B
  • DTH
  • Dmel\\CG10118
  • Pale
  • Ple
  • TH
  • Th
  • VII
  • dTH1
  • dTH65B
  • pale/ple
  • th
  • 14-3-3
  • 14-3-3 epsilon
  • 14-3-3-e
  • 14-3-3-epsilon
  • 14-3-30epsilon
  • 14-3-3EPSILON
  • 14-3-3e
  • 14-3-3omicron
  • 1433epsilon
  • 143E_DROME
  • CG31196
  • CT24092
  • D14-3-3e
  • D14-3-3epsilon
  • Dmel\\CG31196
  • EK3-5
  • PAR-5
  • PAR5
  • Par-5
  • SR3-9
  • Su(Raf)3B
  • anon-WO0172774.141
  • anon-WO02059370.52
  • d14-3-3epsilon
  • eps
  • l(3)j2B10
  • par-5
  • DYT14
  • DYT5b
  • TYH
  • The
Epitope
pSer40
47
36
32
29
18
13
5
5
5
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mammalian
212
186
119
18
14
13
7
6
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
237
50
15
14
8
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
256
159
153
71
46
37
32
26
12
7
4
3
3
2
1
Supplier
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Available images

Specificity Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectiity over dephospho TH.
Purification Affinity-purified
Alternative Name Tyrosine Hydroxylase (TH Antibody Abstract)
Background Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson?s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982, Haycock et al., 1992, Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs. Anti-Phospho Ser40 Tyrosine Hydroxylase Western blot of recombinant phospho- and dephospho-TH showing selective immunolabeling by the phospho-specific antibody of the ~60k TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and dephospho-TH, while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH. Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.
Application Notes Recommended Dilution: WB: 1:1000 IF (frozen sections, Witkovsky et al., 2000): 1:1000 IHC (frozen sections, Witkovsky et al., 2000): 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN361503) Western blots of recombinant phospho- and dephospho-TH showing selective immunolabeli...
 image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN361503) Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and...
Product cited in: Izumi, Ezumi, Takada-Takatori et al.: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, 2014 (PubMed).

McCutcheon, Conrad, Carr et al.: "Dopamine neurons in the ventral tegmental area fire faster in adolescent rats than in adults." in: Journal of neurophysiology, Vol. 108, Issue 6, pp. 1620-30, 2012 (PubMed).

Calipari, Ferris, Melchior et al.: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." in: Addiction biology, Vol. 19, Issue 2, pp. 145-55, 2014 (PubMed).

Salvatore, Pruett: "Dichotomy of Tyrosine Hydroxylase and Dopamine Regulation between Somatodendritic and Terminal Field Areas of Nigrostriatal and Mesoaccumbens Pathways." in: PLoS ONE, Vol. 7, Issue 1, pp. e29867, 2012 (PubMed).

Nakashima, Mori, Kaneko et al.: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." in: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, 2011 (PubMed). (1:3000).

Meyer, Richer, Benkovic et al.: "Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18561-6, 2008 (PubMed).

Jedynak, Ali, Haycock et al.: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, 2002 (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, 2001 (PubMed).

Background publications Dunkley, Bobrovskaya, Graham et al.: "Tyrosine hydroxylase phosphorylation: regulation and consequences." in: Journal of neurochemistry, Vol. 91, Issue 5, pp. 1025-43, 2004 (PubMed).

Kish, Kalasinsky, Derkach et al.: "Striatal dopaminergic and serotonergic markers in human heroin users." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, 2001 (PubMed).

Witkovsky, Gabriel, Haycock et al.: "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina." in: Journal of chemical neuroanatomy, Vol. 19, Issue 2, pp. 105-16, 2000 (PubMed).

Zhu, Klimek, Haycock et al.: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." in: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, 2000 (PubMed).

Zhu, Klimek, Dilley et al.: "Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression." in: Biological psychiatry, Vol. 46, Issue 9, pp. 1275-86, 1999 (PubMed).

Haycock, Bennett, George et al.: "Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine." in: The Journal of biological chemistry, Vol. 257, Issue 22, pp. 13699-703, 1983 (PubMed).

Haycock, Ahn, Cobb et al.: "ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 6, pp. 2365-9, 1992 (PubMed).