Nitrotyrosine antibody

Antigen: Nitrotyrosine

Clonality: Monoclonal (39B6)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN361658

Additional Information

Characteristics: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Immunogen: Hybridoma line 39B6

Format: Liquid

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 39B6

Description: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling . While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury. The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.

Specificity: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific. Species cross-reactivity: Human, Mouse, Rat, Dog.

Sensitivity: 0.7µg/ml of SMC-154 was sufficient for detection of 5µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Concentration: 1 mg/mL

Purification: Protein G purified

Buffer: PBS, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Metabolism, Amino Acids

Restrictions: For Research Use only

Images

Nitrotyrosine (39B6), mouse backskin

Nitrotyrosine (39B6), rat liver