DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody

Antigen: DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1)

Synonyms: DNAJ1, DROJ1, DnaJ1 64EF, EU3500, HSP40, HSP40/HDJ1, Hsp-40, anon-WO0140519.166, anon-WO0172774.135, dHDJ1, dHDJ1/HSP40, dhdj-1, DmelCG10578, CG10578, psiHSP40, hdj1, sis1, hsp40, hspf1, dnajb4, MGC89364, Hsp40, DnaJ-5, MAS5, dnaJ

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN361669

Additional Information

Alternative name: Hsp40

Gene ID: 3337

UniProt: P25685

Immunogen: Recombinant purified human hsp40

Description: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1, is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1. Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK.
Synonyms: DnaJ (Hsp40) homolog subfamily B member 1, DNAJ1, DNAJB1, HDJ1, HSPF1

Characteristics: Accession Number: NP_006136.1

Specificity: Detects an ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Also detects recombinant Hsp40.

Synonyms: DNAJ1, DROJ1, DnaJ1 64EF, EU3500, HSP40, HSP40/HDJ1, Hsp-40, anon-WO0140519.166, anon-WO0172774.135, dHDJ1, dHDJ1/HSP40, dhdj-1, DmelCG10578, CG10578, psiHSP40, hdj1, sis1, hsp40, hspf1, dnajb4, MGC89364, Hsp40, DnaJ-5, MAS5, dnaJ

Application Details

Application Notes: 1: 1500-2,000 (WB)

Purification: Rabbit antiserum

Storage: Store at -20° C. Shipping Temperature: Blue Ice or 4° C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Heat Shock Proteins, Signaling

Restrictions: For Research Use only

Images

Hsp40, heat shocked Hela

Publications

Hattori, Liu, Tohnai et al.: "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." in: Cell structure and function, Vol. 17, Issue 1, pp. 77-86, 1992 (PubMed).

Ohtsuka, Masuda, Nakai et al.: "A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells." in: Biochemical and biophysical research communications, Vol. 166, Issue 2, pp. 642-7, 1990 (PubMed).

Ohki, Tamura, Nishimura et al.: "Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1778-81, 1986 (PubMed).

Bardwell, Tilly, Craig et al.: "The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1782-5, 1986 (PubMed).

Ohtsuka: "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." in: Biochemical and biophysical research communications, Vol. 197, Issue 1, pp. 235-40, 1994 (PubMed).

Melville, Hansen, Freeman et al.: "The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 97-102, 1997 (PubMed).