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DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
|6 references available|
|Price||191.40 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Immunogen||Recombinant purified human hsp40|
DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1, is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1. Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK.
Synonyms: DnaJ (Hsp40) homolog subfamily B member 1, DNAJ1, DNAJB1, HDJ1, HSPF1
|Characteristics||Accession Number: NP_006136.1|
|Specificity||Detects an ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Also detects recombinant Hsp40.|
|Synonyms||DNAJ1, DROJ1, DnaJ1 64EF, EU3500, HSP40, HSP40/HDJ1, Hsp-40, anon-WO0140519.166, anon-WO0172774.135, dHDJ1, dHDJ1/HSP40, dhdj-1, DmelCG10578, CG10578, psiHSP40, hdj1, sis1, hsp40, hspf1, dnajb4, MGC89364, Hsp40, DnaJ-5, MAS5, dnaJ|
|Application Notes||1: 1500-2,000 (WB)|
|Storage||Store at -20° C. Shipping Temperature: Blue Ice or 4° C|
|Storage Shipping Temp Max||Blue Ice or 4 °C|
|Research Area||Heat Shock Proteins, Signaling|
|Restrictions||For Research Use only|
Hattori, Liu, Tohnai et al.: "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." in: Cell structure and function, Vol. 17, Issue 1, pp. 77-86, 1992 (PubMed).
Ohtsuka, Masuda, Nakai et al.: "A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells." in: Biochemical and biophysical research communications, Vol. 166, Issue 2, pp. 642-7, 1990 (PubMed).
Ohki, Tamura, Nishimura et al.: "Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1778-81, 1986 (PubMed).
Bardwell, Tilly, Craig et al.: "The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1782-5, 1986 (PubMed).
Ohtsuka: "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." in: Biochemical and biophysical research communications, Vol. 197, Issue 1, pp. 235-40, 1994 (PubMed).
Melville, Hansen, Freeman et al.: "The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 97-102, 1997 (PubMed).
|Hosts||Rabbit (33), Mouse (26)|
|Reactivities||Human (49), Mouse (Murine) (14), Rat (Rattus) (13), Dog (Canine) (5), Yeast (4), Cow (Bovine) (3), Monkey (3), Chicken (2), Plasmodium falciparum (2), Yeast (Pichia pastoris) (2), Cat (Feline) (1), Fish (1), Guinea Pig (1), Hamster (1), Pig (Porcine) (1), Rabbit (1), Sheep (Ovine) (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (59), ELISA (34), Immunoprecipitation (IP) (18), Immunofluorescence (IF) (17), Immunohistochemistry (IHC) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Flow Cytometry (FACS) (7), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (DB) (1)|
|Conjugates||Biotin (2), FITC (2), HRP (1)|
|Epitopes||C-Term (4), AA 1-340 (3), AA 170-220 (1), N-Term (1)|