DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody

Details for Product No. ABIN361670, Supplier: Log in to see
Antigen
  • DNAJB1P
  • HSP40
  • psiHSP40
  • CG10578
  • DNAJ-1
  • DNAJ1
  • DROJ1
  • Dmel\\CG10578
  • DnaJ1 64EF
  • DroJ1
  • EU3500
  • HSP40/HDJ1
  • Hdj1
  • Hsp-40
  • Hsp40
  • anon-WO0140519.166
  • anon-WO0172774.135
  • dHDJ1
  • dHDJ1/HSP40
  • dHdj1
  • dhdJ1
  • dhdj-1
  • dhdj1
  • dnaJ-1
  • droj1
  • hsp40
  • dnajb4
  • hdj1
  • hspf1
  • sis1
  • DnaJ-5
  • MAS5
  • dnaJ
  • HSPF1
  • RSPH16B
  • Sis1
  • 0610007I11Rik
  • DnaJ (Hsp40) homolog, subfamily B, member 1 pseudogene 1
  • DnaJ-like-1
  • DnaJ (Hsp40) homolog, subfamily B, member 1
  • Hsp40 protein
  • Ydj1p
  • DnaJ protein
  • heat shock protein 40
  • DNAJB1P1
  • DnaJ-1
  • dnajb1
  • Hsp40
  • YDJ1
  • dnaJ
  • hsp40
  • DNAJB1
  • Dnajb1
Reactivity
Human
228
94
91
55
35
15
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11
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2
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2
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1
Host
Rabbit
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157
Clonality
Polyclonal
Conjugate
Un-conjugated
15
15
11
8
8
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7
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7
7
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7
7
7
7
5
5
3
3
3
3
3
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3
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3
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
307
170
139
126
105
79
45
20
7
3
2
1
Options
Supplier
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Supplier Product No.
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Immunogen Recombinant purified human HSP40
Specificity Detects ~40 kDa.
Purification Rabbit Antiserum
Alternative Name HSP40 (DNAJB1 Antibody Abstract)
Background DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (6), is a basic mammalian 40 kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).
Gene ID 3337
NCBI Accession NP_006136
UniProt P25685
Research Area Heat Shock Proteins
Application Notes
  • WB (1:2000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

0.5 μg/ml of SPC-100 was sufficient for detection of HSP40 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Buffer Rabbit Antiserum
Storage -20 °C
Supplier Images
 image for anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody (ABIN361670) Hsp40, heat shocked Hela
Product cited in: Zhong, Ding, Adams, Ghosh, Fu: "Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones." in: Genes & development, Vol. 23, Issue 4, pp. 482-95, 2009 (PubMed).

Background publications Michels, Kanon, Konings, Ohtsuka, Bensaude, Kampinga: "Hsp70 and Hsp40 chaperone activities in the cytoplasm and the nucleus of mammalian cells." in: The Journal of biological chemistry, Vol. 272, Issue 52, pp. 33283-9, 1998 (PubMed).

Melville, Hansen, Freeman, Welch, Katze: "The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 97-102, 1997 (PubMed).

Ohtsuka: "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." in: Biochemical and biophysical research communications, Vol. 197, Issue 1, pp. 235-40, 1994 (PubMed).

Hattori, Liu, Tohnai, Ueda, Kaneda, Kobayashi, Tanabe, Ohtsuka: "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." in: Cell structure and function, Vol. 17, Issue 1, pp. 77-86, 1992 (PubMed).

Ohtsuka, Masuda, Nakai, Nagata: "A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells." in: Biochemical and biophysical research communications, Vol. 166, Issue 2, pp. 642-7, 1990 (PubMed).

Bardwell, Tilly, Craig, King, Zylicz, Georgopoulos: "The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1782-5, 1986 (PubMed).

Ohki, Tamura, Nishimura, Uchida: "Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1778-81, 1986 (PubMed).

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