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p38 antibody (MAP kinase p38)

Details for Product anti-p38 Antibody No. ABIN361679, Supplier: Log in to see
Antigen
Alternatives
anti-Human p38 antibody for Western Blotting
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This p38 antibody is un-conjugated
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1
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Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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89
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29
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24
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11
7
6
2
1
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Supplier
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Immunogen Full length recombinant protein expressed in E.coli cells
Clone 9F12
Specificity Detects ~38 kDa.
Sensitivity Detects ~38kDa protein corresponding to p38alpha MAPK when loaded with 6ng of purified p38alpha by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Alternative Name p38 MAPK (p38 Antibody Abstract)
Background The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).
Cellular Localization: Cytoplasm | Nucleus
Gene ID 1432
NCBI Accession NP_001306
UniProt Q16539
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TCR Signaling, Neurotrophin Signaling Pathway
Application Notes Recommended Dilution: WB (1:1000), IHC (1:1000), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-p38 antibody (MAP kinase p38) (ABIN361679) p38 MAPkinase (9F12), human cell lines.
Background publications Nemes, Petrovski, Aerts et al.: "Transglutaminase-mediated intramolecular cross-linking of membrane-bound alpha-synuclein promotes amyloid formation in Lewy bodies." in: The Journal of biological chemistry, Vol. 284, Issue 40, pp. 27252-64, 2009 (PubMed).

Fan, Chen, Qiao et al.: "Opposing effects of ERK and p38 MAP kinases on HeLa cell apoptosis induced by dipyrithione." in: Molecules and cells, Vol. 23, Issue 1, pp. 30-8, 2007 (PubMed).

Medicherla, Protter, Ma et al.: "Preventive and therapeutic potential of p38 alpha-selective mitogen-activated protein kinase inhibitor in nonobese diabetic mice with type 1 diabetes." in: The Journal of pharmacology and experimental therapeutics, Vol. 318, Issue 1, pp. 99-107, 2006 (PubMed).

Salojin, Owusu, Millerchip et al.: "Essential role of MAPK phosphatase-1 in the negative control of innate immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 3, pp. 1899-907, 2006 (PubMed).

Van Laethem, Van Kelst, Lippens et al.: "Activation of p38 MAPK is required for Bax translocation to mitochondria, cytochrome c release and apoptosis induced by UVB irradiation in human keratinocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 15, pp. 1946-8, 2004 (PubMed).

Deng, Ren, Yang et al.: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003 (PubMed).

Pearson, Robinson, Beers Gibson et al.: "Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions." in: Endocrine reviews, Vol. 22, Issue 2, pp. 153-83, 2001 (PubMed).

Han, Lee, Bibbs et al.: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).