DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (C-Term) antibody

Details for Product No. ABIN361684
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Antigen
Synonyms
DNAJB1P, HSP40, psiHSP40, CG10578, DNAJ-1, DNAJ1, DROJ1, Dmel\\CG10578, DnaJ1 64EF, DroJ1, EU3500, HSP40/HDJ1, Hdj1, Hsp-40, Hsp40, anon-WO0140519.166, anon-WO0172774.135, dHDJ1, dHDJ1/HSP40, dHdj1, d ... show more
DNAJB1P, HSP40, psiHSP40, CG10578, DNAJ-1, DNAJ1, DROJ1, Dmel\\CG10578, DnaJ1 64EF, DroJ1, EU3500, HSP40/HDJ1, Hdj1, Hsp-40, Hsp40, anon-WO0140519.166, anon-WO0172774.135, dHDJ1, dHDJ1/HSP40, dHdj1, dhdJ1, dhdj-1, dhdj1, dnaJ-1, droj1, hsp40, dnajb4, hdj1, hspf1, sis1, DnaJ-5, MAS5, dnaJ, HSPF1, RSPH16B, Sis1, 0610007I11Rik show less
Epitope
C-Term
(1)
Reactivity
Plasmodium falciparum
(3), (2), (2), (1)
Host
Rabbit
(2), (2)
Clonality
Polyclonal
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(4), (4), (4), (3), (2), (2)
Pubmed 9 references available
Quantity 25 μg
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Immunogen C-terminal peptide of Pf11_0513 conjugated to KLH
Specificity Detects ~ 62 kDa. Specific to P. Falciparum and does not cross-react to any protein from Human erythrocytes.
Purification Protein A Affinity Purified
Background DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (1), is a basic mammalian 40 kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the HSP40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
Cellular Localization: Cytoplasm | Nucleus
Gene ID 811045
NCBI Accession XP_001348169
UniProt Q8IHM7
Research Area Signaling, Heat Shock Proteins
Application Notes Recommended Dilution: WB (1:2000), ICC/IF (1:50)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.8 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (C-Term) antibody Hsp40 (Pf11_01513), malarial parasite lysate.
Background publications Pinz, Robbins, Rajasekaran et al.: "Unmasking different mechanical and energetic roles for the small heat shock proteins CryAB and HSPB2 using genetically modified mouse hearts." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 1, pp. 84-92, 2008 (PubMed).

Sugiyama, Suzuki, Kishikawa et al.: "Muscle develops a specific form of small heat shock protein complex composed of MKBP/HSPB2 and HSPB3 during myogenic differentiation." in: The Journal of biological chemistry, Vol. 275, Issue 2, pp. 1095-104, 2000 (PubMed).

Suzuki, Sugiyama, Hayashi et al.: "MKBP, a novel member of the small heat shock protein family, binds and activates the myotonic dystrophy protein kinase." in: The Journal of cell biology, Vol. 140, Issue 5, pp. 1113-24, 1998 (PubMed).

Melville, Hansen, Freeman et al.: "The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 97-102, 1997 (PubMed).

Ohtsuka: "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." in: Biochemical and biophysical research communications, Vol. 197, Issue 1, pp. 235-40, 1994 (PubMed).

Bardwell, Tilly, Craig et al.: "The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1782-5, 1986 (PubMed).

Ohki, Tamura, Nishimura et al.: "Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1778-81, 1986 (PubMed).

Ohtsuka, Masuda, Nakai et al.: "A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells." in: Biochemical and biophysical research communications, Vol. 166, Issue 2, pp. 642-7, 1990 (PubMed).

Hattori, Liu, Tohnai et al.: "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." in: Cell structure and function, Vol. 17, Issue 1, pp. 77-86, 1992 (PubMed).

Validation Images
Catalog No. ABIN361684
224.40 $
Plus shipping costs $45.00
Quantity
Price
25 μg
224.40 $

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