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DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (C-Term) antibody

Details for Product No. ABIN361684, Supplier: Log in to see
Antigen
  • DNAJB1P
  • HSP40
  • psiHSP40
  • CG10578
  • DNAJ-1
  • DNAJ1
  • DROJ1
  • Dmel\\CG10578
  • DnaJ1 64EF
  • DroJ1
  • EU3500
  • HSP40/HDJ1
  • Hdj1
  • Hsp-40
  • Hsp40
  • anon-WO0140519.166
  • anon-WO0172774.135
  • dHDJ1
  • dHDJ1/HSP40
  • dHdj1
  • dhdJ1
  • dhdj-1
  • dhdj1
  • dnaJ-1
  • droj1
  • hsp40
  • dnajb4
  • hdj1
  • hspf1
  • sis1
  • DnaJ-5
  • MAS5
  • dnaJ
  • HSPF1
  • RSPH16B
  • Sis1
  • 0610007I11Rik
  • DnaJ (Hsp40) homolog, subfamily B, member 1 pseudogene 1
  • DnaJ-like-1
  • DnaJ (Hsp40) homolog, subfamily B, member 1
  • Hsp40 protein
  • Ydj1p
  • DnaJ protein
  • heat shock protein 40
  • DNAJB1P1
  • DnaJ-1
  • dnajb1
  • Hsp40
  • YDJ1
  • dnaJ
  • hsp40
  • DNAJB1
  • Dnajb1
Epitope
C-Term
54
42
2
2
2
2
2
2
1
1
1
1
Reactivity
Plasmodium falciparum
219
96
94
42
35
16
14
12
8
6
5
5
5
4
4
4
3
3
3
2
2
2
1
Host
Rabbit
164
138
Clonality
Polyclonal
Conjugate
Un-conjugated
14
14
10
8
8
7
7
7
7
7
7
7
7
7
7
7
5
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
287
151
130
130
92
69
31
21
7
3
2
1
Options
Supplier
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Immunogen C-terminal peptide of Pf11_0513 conjugated to KLH
Specificity Detects ~ 62 kDa. Specific to P. Falciparum and does not cross-react to any protein from Human erythrocytes.
Purification Protein A Purified
Alternative Name HSP40 (DNAJB1 Antibody Abstract)
Background DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (1), is a basic mammalian 40 kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the HSP40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
Gene ID 811046
NCBI Accession XP_001348169
UniProt Q8IHM7
Research Area Heat Shock Proteins
Application Notes
  • WB (1:2000)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

0.9 μg/ml of SPC-184 was sufficient for detection of Pf11_0513 in 20 μg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1.8 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (C-Term) antibody (ABIN361684) Hsp40 (Pf11_01513), malarial parasite lysate.
Background publications Pinz, Robbins, Rajasekaran, Benjamin, Ingwall: "Unmasking different mechanical and energetic roles for the small heat shock proteins CryAB and HSPB2 using genetically modified mouse hearts." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 1, pp. 84-92, 2008 (PubMed).

Sugiyama, Suzuki, Kishikawa, Akutsu, Hirose, Waye, Tsui, Yoshida, Ohno: "Muscle develops a specific form of small heat shock protein complex composed of MKBP/HSPB2 and HSPB3 during myogenic differentiation." in: The Journal of biological chemistry, Vol. 275, Issue 2, pp. 1095-104, 2000 (PubMed).

Suzuki, Sugiyama, Hayashi, Nyu-i, Yoshida, Nonaka, Ishiura, Arahata, Ohno: "MKBP, a novel member of the small heat shock protein family, binds and activates the myotonic dystrophy protein kinase." in: The Journal of cell biology, Vol. 140, Issue 5, pp. 1113-24, 1998 (PubMed).

Melville, Hansen, Freeman, Welch, Katze: "The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 97-102, 1997 (PubMed).

Ohtsuka: "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." in: Biochemical and biophysical research communications, Vol. 197, Issue 1, pp. 235-40, 1994 (PubMed).

Hattori, Liu, Tohnai, Ueda, Kaneda, Kobayashi, Tanabe, Ohtsuka: "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." in: Cell structure and function, Vol. 17, Issue 1, pp. 77-86, 1992 (PubMed).

Ohtsuka, Masuda, Nakai, Nagata: "A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells." in: Biochemical and biophysical research communications, Vol. 166, Issue 2, pp. 642-7, 1990 (PubMed).

Bardwell, Tilly, Craig, King, Zylicz, Georgopoulos: "The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1782-5, 1986 (PubMed).

Ohki, Tamura, Nishimura, Uchida: "Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1778-81, 1986 (PubMed).