Hsp40 (Pf11_0513) antibody

Antigen: Hsp40 (Pf11_0513)

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN361684

Additional Information

Gene ID: 811045

UniProt: Q8IHM7

Immunogen: C-terminal peptide of Pf11_0513 conjugated to KLH

Description: HSPB2, also known as MKBP is the most divergent member of the sHSP family with only 30% sequence identity to all other mammalian sHSPS. MKBP is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. MKBP enhances the kinase activity of DMPK and protects it from heat-induced activation. MKBP also shows a unique nature compared to other sHSps, in that the expression of MKBP is not induced by heat shock. In unstressed skeletal muscle, MKBP forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction. During stress, these complexes dissolve and MKBP’s localization to mitochondria increases, leading to increased cell survival. Pinz et al. tried to find a distinct role for MKBP in terms of cardiac mechanics and finds that it is required for normal systolic performance and normal cardiac energetic. HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, suggesting that MKBP may be an important factor in tumor transformation and metastasis.
Synonyms: PF11_0513

Characteristics: Accession Number: XP_001348169.2

Specificity: Detects an 62kD protein, corresponding to P.falciparum Hsp40 on SDS PAGE immunoblot, in samples from P.Falciparum origin.
It is species specific to P.falciparum, does not cross-rect to any protein from human erythrocytes.

Application Details

Application Notes: 1:2000 (WB), 1:50 (IF)

Concentration: 1.8mg/mL

Purification: Protein A Affinity Purified

Buffer: PBS pH7.4, 50% glycerol

Preservative: 0.09% sodium azide

Storage: Store at -20° C. Shipping Temperature: Blue Ice or 4° C

Research Area: Heat Shock Proteins

Restrictions: For Research Use only

Images

Hsp40 (Pf11_01513), malarial parasite lysate.

Publications

Hattori, Liu, Tohnai et al.: "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." in: Cell structure and function, Vol. 17, Issue 1, pp. 77-86, 1992 (PubMed).

Ohtsuka, Masuda, Nakai et al.: "A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells." in: Biochemical and biophysical research communications, Vol. 166, Issue 2, pp. 642-7, 1990 (PubMed).

Ohki, Tamura, Nishimura et al.: "Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1778-81, 1986 (PubMed).

Bardwell, Tilly, Craig et al.: "The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1782-5, 1986 (PubMed).

Ohtsuka: "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." in: Biochemical and biophysical research communications, Vol. 197, Issue 1, pp. 235-40, 1994 (PubMed).

Melville, Hansen, Freeman et al.: "The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 97-102, 1997 (PubMed).