DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody

Antigen: DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1)

Synonyms: DNAJ1, DROJ1, DnaJ1 64EF, EU3500, HSP40, HSP40/HDJ1, Hsp-40, anon-WO0140519.166, anon-WO0172774.135, dHDJ1, dHDJ1/HSP40, dhdj-1, DmelCG10578, CG10578, psiHSP40, hdj1, sis1, hsp40, hspf1, dnajb4, MGC89364, Hsp40, DnaJ-5, MAS5, dnaJ

Clonality: Polyclonal

Host: Rabbit

Reactivity: Plasmodium falciparum

Catalog no.: ABIN361685

Additional Information

Alternative name: Hsp40

Immunogen: C-terminal peptide of Pf11_0512 conjugated to KLH

Format: Protein A purified

Description: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the Hsp40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.

Specificity: This protein A Affinity purified antibody detects an 62kDa protein, corresponding to P. falciparum Hsp40 on SDS PAGE immunoblot, in samples from P. falciparum origin. Species cross-reactivity: It is species specific to P. falciparum and does not cross-react to any protein from human erythrocytes.

Synonyms: DNAJ1, DROJ1, DnaJ1 64EF, EU3500, HSP40, HSP40/HDJ1, Hsp-40, anon-WO0140519.166, anon-WO0172774.135, dHDJ1, dHDJ1/HSP40, dhdj-1, DmelCG10578, CG10578, psiHSP40, hdj1, sis1, hsp40, hspf1, dnajb4, MGC89364, Hsp40, DnaJ-5, MAS5, dnaJ

Application Details

Application Notes: 1:2000 dilution for western blot and a 1:50 dilution for IF. Higher dilutions can be tried.

Concentration: 1.8mg/ml

Buffer: PBS pH 7.4, 50% glycerol, 0.09% azide. Protein A affinity purified.

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: Signaling, Heat Shock Proteins

Restrictions: For Research Use only

Images

Hsp40 (Pf11_01513), malarial parasite lysate