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Lysine (lys) (methylated) antibody

lys WB, ICC, IF, IP, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5650776
  • Target
    Lysine (lys)
    Binding Specificity
    • 23
    • 10
    methylated
    Reactivity
    Please inquire
    Host
    • 21
    • 12
    Rabbit
    Clonality
    • 21
    • 12
    Polyclonal
    Conjugate
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Un-conjugated
    Application
    • 33
    • 32
    • 32
    • 32
    • 32
    • 11
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA
    Specificity
    Detects proteins containing methylated lysine residues.
    Purification
    Protein A Purified
    Immunogen
    Methylated KLH Conjugated
  • Application Notes
    • WB (1:20000)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    Comment

    0.2-0.5 μg/ml of SPC-158 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, Storage buffer may change when conjugated
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Mattice, MacLean, Childers, Storey: "-Characterization of pyruvate kinase from the anoxia tolerant turtle, Trachemys scripta elegans: a potential role for enzyme methylation during metabolic rate depression." in: PeerJ, Vol. 6, pp. e4918, (2018) (PubMed).

    Dawson, Bell, Storey: "Purification and Properties of White Muscle Lactate Dehydrogenase from the Anoxia-Tolerant Turtle, the Red-Eared Slider, Trachemys scripta elegans." in: Enzyme research, Vol. 2013, pp. 784973, (2013) (PubMed).

    Shahriari, Dawson, Bell, Storey: "Stable Suppression of Lactate Dehydrogenase Activity during Anoxia in the Foot Muscle of Littorina littorea and the Potential Role of Acetylation as a Novel Posttranslational Regulatory Mechanism." in: Enzyme research, Vol. 2013, pp. 461374, (2013) (PubMed).

  • Target
    Lysine (lys)
    Alternative Name
    Lysine
    Synonyms
    lysine antibody, lys antibody
    Target Type
    Amino Acid
    Background
    Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
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