Phosphoserine antibody (HRP)

Details for Product No. ABIN361695, Supplier: Log in to see
Antigen
Reactivity
Chemical
117
4
3
1
1
Host
Rabbit
77
44
Clonality
Polyclonal
Conjugate
HRP
11
10
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
100
74
67
36
22
18
13
10
8
1
1
1
1
Options
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Immunogen Phosphoserine conjugated to KLH, and phosvitin mixture
Specificity Detects proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Purification Protein A Purified
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
Research Area Amino Acids, Protein Modifications, Metabolism, Neurology
Application Notes
  • WB (1:500)
  • ICC/IF (1:50)
  • ELISA (1:250)
  • IP (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

2 μg/ml of SPC-151 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 μM okadaic acid.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer HRP Stabilizing buffer
Storage 4 °C
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Phosphoserine antibody (HRP) (ABIN361695) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphoserine Polyc...
Western Blotting (WB) image for anti-Phosphoserine antibody (HRP) (ABIN361695) Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine prot...
Background publications Goto, Kiyono, Tomono, Kawajiri, Urano, Furukawa, Nigg, Inagaki: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).

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