Ribosomal Protein S6 Kinase, Polypeptide 1 (RPS6KB1) antibody

Antigen: Ribosomal Protein S6 Kinase, Polypeptide 1 (RPS6KB1)

Synonyms: 7084, DS6K, Dp70S6k, Dp70[s6k], S6K, dps6k, fs(3)07084, l(3)07084, p70 S6K, p70/S6K, p70S6K, p70[S6 kinase], p70[S6k], p70[S6kinase], s6k11, DmelCG10539, CG10539, S6K1, 70kDa, p70s6k, AA959758, AI256796, AI314060, p70/85s6k, 2610318I15Rik, 4732464A07Rik, p70s6k-A, pkacb, MGC80071, XPKAbeta

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Guinea Pig, Cow (Bovine)

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Catalog no.: ABIN361700

Additional Information

Characteristics: Detects a ~70kDa protein corresponding to the molecular mass of p70 S6K on SDS PAGE immunoblots.

Alternative name: p70 S6K

Gene ID: NP_003943.2, 6199

Immunogen: Human p70 S6K c-terminal peptide –KLH conjugates

Format: Liquid

Description: Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5’ transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.
Synonyms: p70 S6 kinase beta (phospho T229), p70-beta, p70S6Kb, S6K beta, S6K2, SRK, STK14B

Specificity: Detects a ~70kDa protein, corresponding to the molecular mass of p70 S6K on SDS-PAGE immunoblots. Species cross-reactivity: Human, Mouse, Rat, Bovine.

Synonyms: 7084, DS6K, Dp70S6k, Dp70[s6k], S6K, dps6k, fs(3)07084, l(3)07084, p70 S6K, p70/S6K, p70S6K, p70[S6 kinase], p70[S6k], p70[S6kinase], s6k11, DmelCG10539, CG10539, S6K1, 70kDa, p70s6k, AA959758, AI256796, AI314060, p70/85s6k, 2610318I15Rik, 4732464A07Rik, p70s6k-A, pkacb, MGC80071, XPKAbeta

Application Details

Application Notes: 1ug/mL was needed for western blot analysis

Concentration: 250µg/mL

Buffer: TBS, 50% glycerol, 0.09% sodium azide

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cell Signaling

Restrictions: For Research Use only

Images

p70 S6K, mouse brain (R), neg CT (L)

Publications

Publications: Kawasome, Papst, Webb et al.: "Targeted disruption of p70(s6k) defines its role in protein synthesis and rapamycin sensitivity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5033-8, 1998 (PubMed).

Gout, Minami, Hara et al.: "Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region." in: The Journal of biological chemistry, Vol. 273, Issue 46, pp. 30061-4, 1998 (PubMed).

Shima, Pende, Chen et al.: "Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase." in: The EMBO journal, Vol. 17, Issue 22, pp. 6649-59, 1999 (PubMed).

Boyer, Quintanilla, Lee-Fruman: "Regulation of catalytic activity of S6 kinase 2 during cell cycle." in: Molecular and cellular biochemistry, Vol. 307, Issue 1-2, pp. 59-64, 2007 (PubMed).