Inhibitor of Growth Family, Member 1 (ING1) antibody

Antigen: Inhibitor of Growth Family, Member 1 (ING1)

Synonyms: p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, p33ING1c, ING1, MGC136918, zgc:136918

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Catalog no.: ABIN361701

Additional Information

Characteristics: Detects a ~33kDa protein corresponding to the molecular mass of p33 ING1 on SDS PAGE immunoblots.

Alternative name: p33 ING1

Gene ID: NP_036049.2, 26356

Immunogen: Mouse p33 ING1 N-terminal peptide –KLH conjugates

Format: Liquid

Description: The p33ING1 protein encodes a 33-kD, 294-amino acid protein that displays the characteristics of a tumor suppressor gene. The p33ING1 protein is a regulator of cell cycle, senescence, and apoptosis. It can mediate growth arrest, anchor dependent growth and influence chemosensitivity (1-4). It also has a proposed role as a growth regulator, which is consistent with its location in the nucleus (5). It has been demonstrated that the ING1 gene has three exons and that four mRNA variants are transcribed from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 IG1) (6, 7). ING1 can possibly influence the above biological functions depending on which variant is expressed (1-4).
Synonyms: growth inhibitor ING1, ING1, inhibitor of growth 1, p24ING1c, p33, p33ING, p33ING1b, p33ING1c, p47, p47ING1a, tumor supressor ING1

Specificity: Detects a ~33kDA protein, corresponding to the apparent molecular mass of p33 ING1 on SDS-PAGE immunoblots. Species cross-reactivity: Human (1) and Mouse. Not cross-reactive with any other ING family.

Application Details

Application Notes: 1ug/mL was needed for western blot analysis.

Concentration: 250µg/mL

Buffer: TBS, 50% glycerol, 0.09% sodium azide

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cell Signaling

Restrictions: For Research Use only

Images

p33 ING1, mouse brain extract

Publications

Publications: Garkavtsev, Kazarov, Gudkov et al.: "Suppression of the novel growth inhibitor p33ING1 promotes neoplastic transformation." in: Nature genetics, Vol. 14, Issue 4, pp. 415-20, 1997 (PubMed).

Jaeger, Stockert, Scanlan et al.: "Cancer-testis antigens and ING1 tumor suppressor gene product are breast cancer antigens: characterization of tissue-specific ING1 transcripts and a homologue gene." in: Cancer research, Vol. 59, Issue 24, pp. 6197-204, 2000 (PubMed).

Gunduz, Ouchida, Fukushima et al.: "Genomic structure of the human ING1 gene and tumor-specific mutations detected in head and neck squamous cell carcinomas." in: Cancer research, Vol. 60, Issue 12, pp. 3143-6, 2000 (PubMed).

Cheung, Li: "The tumor suppressor ING1: structure and function." in: Experimental cell research, Vol. 268, Issue 1, pp. 1-6, 2001 (PubMed).

Cheung, Li: "The tumour suppressor p33ING1 does not enhance camptothecin-induced cell death in melanoma cells." in: International journal of oncology, Vol. 20, Issue 6, pp. 1319-22, 2002 (PubMed).

Cheung, Li: "p33(ING1) enhances UVB-induced apoptosis in melanoma cells." in: Experimental cell research, Vol. 279, Issue 2, pp. 291-8, 2002 (PubMed).

Cheung, Li: "The tumour suppressor p33ING1 does not regulate migration and angiogenesis in melanoma cells." in: International journal of oncology, Vol. 21, Issue 6, pp. 1361-5, 2002 (PubMed).