Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) antibody

Antigen: Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1)

Synonyms: RSK, HU-1, RSK1, MAPKAPK1A

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Guinea Pig, Cow (Bovine)

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Catalog no.: ABIN361702

Additional Information

Characteristics: Detects a ~90kDa protein corresponding to the molecular mass of p90 RSK1 on SDS PAGE immunoblots.

Alternative name: p90 RSK1

Gene ID: NP_002944.2, 6195

Immunogen: Human p90 RSK1 C-terminal peptide, conjugated to KLH

Format: Liquid

Description: The p90 ribosomal S6 kinase (Rsk) family comprises four mammalian serine/threonine kinases (Rsk1–4). In the past, S6K1 and S6K2 were thought to be the predominant operating S6 kinases, however RSK 1 and 2 have been shown to phosphorylate S6 in response to the ERK pathway, or otherwise known as the extra cellular signal-regulated kinases pathway. RSK 1 in particular is very multifunctional as it participates in nuclear signaling, regulates nuclear factors, regulates several transcription factors like c-Fos, and interacts with the transcriptional coactivator CREB-binding protein. As a result, RSK1 seems to have an important role in cellular growth control and proliferation. (1-5).
Synonyms: RSK1 p90, HU1, MAPKAP, p90rsk, p90S6K, RSK1, RSK 1 p90

Specificity: Detects an ~90kDa protein, corresponding to the molecular mass of p90 RSK1 on SDS-PAGE immunoblots. Species cross-reactivity: Human, Mouse, Rat, Bovine.

Application Details

Application Notes: 1ug/ml was needed for western blot analysis.

Concentration: 250µg/mL

Buffer: TBS, 50% glycerol, 0.09% sodium azide

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cell Signaling

Restrictions: For Research Use only

Images

P90 RSK1, IP and WB, mouse brain extract (A) and (B) neg IP control

Publications

Publications: Joel, Smith, Sturgill et al.: "pp90rsk1 regulates estrogen receptor-mediated transcription through phosphorylation of Ser-167." in: Molecular and cellular biology, Vol. 18, Issue 4, pp. 1978-84, 1998 (PubMed).

De Cesare, Jacquot, Hanauer et al.: "Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 21, pp. 12202-7, 1998 (PubMed).

Merienne, Jacquot, Trivier et al.: "Rapid immunoblot and kinase assay tests for a syndromal form of X linked mental retardation: Coffin-Lowry syndrome." in: Journal of medical genetics, Vol. 35, Issue 11, pp. 890-4, 1999 (PubMed).

Froedin, Gammeltoft: "Role and regulation of 90 kDa ribosomal S6 kinase (RSK) in signal transduction." in: Molecular and cellular endocrinology, Vol. 151, Issue 1-2, pp. 65-77, 1999 (PubMed).