Hsp90beta antibody

Antigen: Hsp90beta

Clonality: Monoclonal (Hyb-K3701)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN361720

Additional Information

Characteristics: Detects 90kDa proteins corresponding to the molecular mass of Hsp90beta.

Gene ID: 3326

Swiss-Prot: P08238

Immunogen: Recombinant human Hsp90beta

Format: Liquid

Isotype: IgM  (Matching secondary antibodies)

Clone: Hyb-K3701

Description: HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment. Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta exists mainly as a monomer. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. Furthermore, Hsp90 is highly conserved between species, having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling. The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function.
Synonyms: Hsp84, Hsp90B, HspC2, HspCB, D6S182. FLJ26984

Specificity: Detects 90kD proteins corresponding to the molecular mass of hsp90beta. Species cross-reactivity: Human (beta-specific). Does not cross-react with alpha. Rat.

Sensitivity: 1µg/ml was sufficient for detection of Hsp90beta in 20µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Application Details

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.2, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

Hsp90beta (K3701), Mouse colon 1 in 3000 Amplifier

Hsp90beta (K3701), Mouse colon 1 in 3000, Amplifier