AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2)

Details for Product anti-AKT2 Antibody No. ABIN361723, Supplier: Log in to see
Antigen
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • AKT2
  • akt2
  • Akt2
Alternatives
anti-Human AKT2 antibody for ELISA
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen A five residue synthetic peptide based on the human Akt2, coupled to KLH
Specificity Detects ~65 kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).
Purification Protein A Purified
Alternative Name AKT2 (AKT2 Antibody Abstract)
Background Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
Gene ID 208
NCBI Accession NP_001617
UniProt P31751
Research Area Atherosclerosis, Signaling, Cardiovascular
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
Application Notes
  • WB (1:1000)
  • ICC/IF (1:25)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody (ABIN361723) Akt2(PKBb), cell line mix_1.
Background publications Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002 (PubMed).

Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001 (PubMed).

Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998 (PubMed).

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