V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2)

Synonyms: PKBB, PRKBB, PKBBETA, RAC-BETA, AKT2, cb945, prkbb, v-akt2, pkbbeta, rac-beta

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Dog (Canine), Guinea Pig, Hamster, Monkey, Pig (Porcine), Rabbit, Sheep (Ovine), Xenopus laevis

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN361723

Additional Information

Alternative name: Akt2 (PKBbeta)

Immunogen: A five residue synthetic peptide based on the human Akt2, coupled to KLH

Description: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).

Specificity: Detects a ~65kDa protein, corresponding to the molecular mass of Akt2 PKB beta on SDS-PAGE immunoblots. Does not react with PKBa (Akt1) or PKBb (Akt3). Species cross-reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus.

Application Details

Application Notes: 1:3000 dilution of SPC-148 was sufficient for detection of PKB

Concentration: 0.25mg/mL

Buffer: Rabbit immunoglobulin in 0.1M Tris-acetate buffer, 0.1% sodium azide.

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Restrictions: For Research Use only

Images

Akt2(PKBb), rat tissues

Publications

Publications: Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998 (PubMed).

Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001 (PubMed).

Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002 (PubMed).