V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody

Details for Product No. ABIN361723
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Antigen
Synonyms AKT2, pkbb, pkbbeta, pkbss, prkbb, rac-beta, v-akt2, 2410016A19Rik, AW554154, PKB, PKBbeta, HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, cb945, akt2-a
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 3 references available
Quantity 400 μL
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Immunogen A five residue synthetic peptide based on the human Akt2, coupled to KLH
Specificity Detects ~65 kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).
Purification Rabbit immunoglobulin
Background Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
Gene ID 208
NCBI Accession NP_001617
UniProt P31751
Research Area Cell Signaling, Phosphorylation
Application Notes Recommended Dilution: WB (1:3000)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody Akt2(PKBb), cell line mix_1.
Background publications Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002 (PubMed).

Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001 (PubMed).

Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998 (PubMed).

Validation Images
Catalog No. ABIN361723
344.30 $
Plus shipping costs $45.00
Quantity
Price
400 μL
344.30 $

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