Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody

Antigen

V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2)

Synonyms PKBB, PRKBB, PKBBETA, RAC-BETA, AKT2, cb945, prkbb, v-akt2, pkbbeta, rac-beta
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Dog (Canine), Guinea Pig, Hamster, Monkey, Pig (Porcine), Rabbit, Sheep (Ovine), Xenopus laevis

Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
3 references available
Catalog no. ABIN361723
Quantity 400µl  (0.25mg/mL)  (Variants)
Price 319.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 3 to 4 Business Days

Additional Information

Alternative name Akt2 (PKBbeta)
Gene ID 208
UniProt P31751
Immunogen A five residue synthetic peptide based on the human Akt2, coupled to KLH
Description Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2. Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target. Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration.
Synonyms: AKT2, PKB, PKB beta, PRKBB, protein kinase Akt2, RAC BETA
Characteristics Accession Number: NP_001617.1
Specificity Detects a 65kDa protein, corresponding to the molecular mass of Akt2 PKBbeta on SDS PAGE immunoblots. Does not react with PKBalpha (Akt1) or PKBgamma (Akt3).

Application Details

Application Notes 1:3000 dilution was sufficient for detection of PKBbeta in 20µg of HeLa cell lysate by ECL immunoblot analysis.
Concentration 0.25mg/mL
Purification Rabbit immunoglobulin
Buffer TBS, 50% glycerol
Preservative 0.09% sodium azide
Storage Store at -20° C. Shipping Temperature: Blue Ice or 4° C
Storage Shipping Temp Max Blue Ice or 4 °C
Research Area Cell Signaling, Phosphorylation
Restrictions For Research Use only

Publications

Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998 (PubMed).

Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001 (PubMed).

Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002 (PubMed).

Alternatives

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2)", type "Antibodies"
Hosts Rabbit (98), Mouse (26), Rat (3), Goat (2), Chicken (1)
Reactivities Human (127), Rat (Rattus) (57), Mouse (Murine) (54), Dog (Canine) (10), Cow (Bovine) (9), Chicken (8), Pig (Porcine) (7), Sheep (Ovine) (6), Monkey (5), Xenopus laevis (2), Cat (Feline) (1), Guinea Pig (1), Hamster (1), Primate (1), Rabbit (1), Zebrafish (1)
Applications Western Blotting (WB) (103), ELISA (64), Immunofluorescence (IF) (34), Immunohistochemistry (IHC) (28), Flow Cytometry (FACS) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunoprecipitation (IP) (12), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Agarose Beads (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer474 (14), C-Term (9), Internal Region (5), N-Term (4), Ser474 (4), AA 455-468 (3), AA 455-469 (3), AA 2-481 (2), AA 106-123 (1), Center (1)