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TLR4 antibody (Toll-Like Receptor 4) (AA 420-435)

Details for Product anti-TLR4 Antibody No. ABIN361724, Supplier: Log in to see
Antigen
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll-like receptor 4
  • TLR4
  • Tlr4
Alternatives
anti-Human TLR4 antibody for Flow Cytometry
Epitope
AA 420-435
41
37
30
27
16
16
16
15
14
13
13
12
11
10
8
6
5
5
5
5
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
502
246
141
75
51
50
43
26
12
10
4
2
2
1
1
Host
Rabbit
283
254
55
10
Clonality
Polyclonal
Conjugate
This TLR4 antibody is un-conjugated
49
43
40
16
13
12
12
8
8
8
7
7
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
370
294
134
131
123
107
96
96
34
27
26
24
21
16
12
8
8
4
4
3
3
2
2
1
1
1
1
Options
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Immunogen Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Specificity Detects ~75-80 kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Purification Protein A Purified
Alternative Name TLR4 (TLR4 Antibody Abstract)
Background The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa (6).
Gene ID 7099
NCBI Accession NP_612564
UniProt O00206
Research Area Immunology, Innate Immunity, Adaptive Immunity, Atherosclerosis, Signaling, Inflammation
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin
Application Notes
  • WB (1:500)
  • IHC (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

2 μg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-TLR4 antibody (Toll-Like Receptor 4) (AA 420-435) (ABIN361724) TLR4 MD 2 recomb, 95kD.
 image for anti-TLR4 antibody (Toll-Like Receptor 4) (AA 420-435) (ABIN361724) anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Image 2)
Product cited in: Nakano, Furube, Morita, Wanaka, Nakashima, Miyata: "Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain." in: Journal of neuroimmunology, Vol. 278, pp. 144-58, 2015 (PubMed).

Takano, Ohkusa, Otani, Min, Kadoyama, Minami, Sano, Matsuyama: "Lipid A-activated inducible nitric oxide synthase expression via nuclear factor-κB in mouse choroid plexus cells." in: Immunology letters, Vol. 167, Issue 2, pp. 57-62, 2015 (PubMed).

Background publications Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).

Poltorak, Ricciardi-Castagnoli, Citterio, Beutler: "Physical contact between lipopolysaccharide and toll-like receptor 4 revealed by genetic complementation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2163-7, 2000 (PubMed).

Takeuchi, Hoshino, Kawai, Sanjo, Takada, Ogawa, Takeda, Akira: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Muzio, Natoli, Saccani, Levrero, Mantovani: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Medzhitov, Preston-Hurlburt, Janeway: "A human homologue of the Drosophila Toll protein signals activation of adaptive immunity." in: Nature, Vol. 388, Issue 6640, pp. 394-7, 1997 (PubMed).

Kammeraat, Veldstra: "Characterization of succinate semialdehyde dehydrogenase from rat brain." in: Biochimica et biophysica acta, Vol. 151, Issue 1, pp. 1-10, 1970 (PubMed).