You are viewing an incomplete version of our website. Please click to reload the website as full version.

DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody

Details for Product No. ABIN361726, Supplier: Login to see
Antigen
  • cb1075
  • cb983
  • dmnt1
  • fb05h01
  • mta
  • wu:fb05h01
  • ADCADN
  • AIM
  • CXXC9
  • DNMT
  • HSN1E
  • MCMT
  • DDM2
  • DECREASED DNA METHYLATION 2
  • DMT01
  • DMT1
  • DNA METHYLTRANSFERASE
  • DNA METHYLTRANSFERASE 01
  • DNA METHYLTRANSFERASE 1
  • MET2
  • METHYLTRANSFERASE 2
  • METHYLTRANSFERASE I
  • METI
  • methyltransferase 1
  • Cxxc9
  • Dnmt
  • Dnmt1o
  • MTase
  • Met-1
  • Met1
  • MommeD2
  • m.MmuI
Epitope
AA 637-650
58
42
35
28
22
22
20
19
13
13
13
12
10
9
9
7
7
7
6
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Fish, Human, Mouse (Murine), Zebrafish (Danio rerio)
387
109
41
39
35
5
5
5
3
2
1
1
1
1
1
1
Host
Mouse
252
110
20
8
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
23
23
20
18
18
18
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
Application
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
255
130
103
96
81
72
46
39
26
22
10
7
2
1
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
Clone 60B1220-1
Specificity Detects ~180 kDa. It will cross-react with mouse DNMT1.
Purification Protein G Purified
Alternative Name DNMT1 (DNMT1 Antibody Abstract)
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
Cellular Localization: Cytoplasm | Endoplasmic Reticulum
Gene ID 1786
NCBI Accession NP_001370
UniProt P26358
Research Area DNA/RNA
Pathways
Application Notes Recommended Dilution: WB (1:500), IHC (1:500), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.05 % BSA, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody (ABIN361726) Dnmt1, 18ug H1299 lysate 1 in1000 Western Blotting.
Background publications Robertson, Ait-Si-Ali, Yokochi et al.: "DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters." in: Nature genetics, Vol. 25, Issue 3, pp. 338-42, 2000 (PubMed).

Reik, Kelsey, Walter: "Dissecting de novo methylation." in: Nature genetics, Vol. 23, Issue 4, pp. 380-2, 1999 (PubMed).

Okano, Bell, Haber et al.: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).

Xie, Wang, Okano et al.: "Cloning, expression and chromosome locations of the human DNMT3 gene family." in: Gene, Vol. 236, Issue 1, pp. 87-95, 1999 (PubMed).

Bestor, Laudano, Mattaliano et al.: "Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases." in: Journal of molecular biology, Vol. 203, Issue 4, pp. 971-83, 1989 (PubMed).

Yen, Vertino, Nelkin et al.: "Isolation and characterization of the cDNA encoding human DNA methyltransferase." in: Nucleic acids research, Vol. 20, Issue 9, pp. 2287-91, 1992 (PubMed).